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◦c with fcblock  (Miltenyi Biotec)


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    Miltenyi Biotec ◦c with fcblock
    ◦C With Fcblock, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 3318 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%E2%97%A6c+with+fcblock/FcR+Blocking+Reagent%2C+human/pm36115212-55-23-26
    Average 97 stars, based on 3318 article reviews
    ◦c with fcblock - by Bioz Stars, 2026-10
    97/100 stars

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    Staining:

    Article Title: Antigen-specific immune reactions by expanded CD8 + T cell clones from HLA-B*27-positive patients with spondyloarthritis.
    Article Snippet: PBMC and SF cells were subjected to purification by Ficoll-Paque gradient centrifugation, subsequent erythrocyte-lysis and washed with PBS. .. After washing, cells were either frozen alive at − 80 ◦C or directly stained and sorted using the following procedure: 10min at 4 ◦C with Fcblock (Miltenyi #130-059-901) at 1/50 dilution in FACS buffer (PBS with 2% FBS), followed by the surface antibody staining mix at a total of 100μl FACS buffer for 30min at 4 ◦C: anti-human CD45RO-FITC (#11- 0457-42) at 1/40 dilution, anti-human CCR7-APC (#17-1979-42) at 1/ 40 dilution, anti-human CD3-AF700 (#56-0037-42) at 1/50 dilution, Fixable Viability Dye eFluorTM 780 (#65-0865-14) at 1/1000, antihuman CD4-Pacific Blue (MHCD0428) at 1/25, all purchased from ThermoFisher, as well as anti-human CD8-PerCP (344708) at 1/50 from BioLegend. .. Antigen-experienced CD8+ T cells consisting of effector memory (CD45RO+, CCR7-), effector (CD45RO− , CCR7-) and central memory (CD45RO+, CCR7+) cells were collected using a FACS Aria Fusion flow cytometer (BD Biosciences).

    FACS:

    Article Title: Antigen-specific immune reactions by expanded CD8 + T cell clones from HLA-B*27-positive patients with spondyloarthritis.
    Article Snippet: PBMC and SF cells were subjected to purification by Ficoll-Paque gradient centrifugation, subsequent erythrocyte-lysis and washed with PBS. .. After washing, cells were either frozen alive at − 80 ◦C or directly stained and sorted using the following procedure: 10min at 4 ◦C with Fcblock (Miltenyi #130-059-901) at 1/50 dilution in FACS buffer (PBS with 2% FBS), followed by the surface antibody staining mix at a total of 100μl FACS buffer for 30min at 4 ◦C: anti-human CD45RO-FITC (#11- 0457-42) at 1/40 dilution, anti-human CCR7-APC (#17-1979-42) at 1/ 40 dilution, anti-human CD3-AF700 (#56-0037-42) at 1/50 dilution, Fixable Viability Dye eFluorTM 780 (#65-0865-14) at 1/1000, antihuman CD4-Pacific Blue (MHCD0428) at 1/25, all purchased from ThermoFisher, as well as anti-human CD8-PerCP (344708) at 1/50 from BioLegend. .. Antigen-experienced CD8+ T cells consisting of effector memory (CD45RO+, CCR7-), effector (CD45RO− , CCR7-) and central memory (CD45RO+, CCR7+) cells were collected using a FACS Aria Fusion flow cytometer (BD Biosciences).



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    ◦c with fcblock  (Miltenyi Biotec)
    97
    Miltenyi Biotec ◦c with fcblock
    ◦C With Fcblock, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%E2%97%A6c+with+fcblock/FcR+Blocking+Reagent%2C+human/pm36115212-55-23-26
    Average 97 stars, based on 1 article reviews
    ◦c with fcblock - by Bioz Stars, 2026-10
    97/100 stars
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